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The reverse genetic strategy along with a tracing experiment utilizing secure isotope-labeled fatty acids unveiled that the ELO/DES pathway is the one DHA synthesis pathway in Parietichytrium sp. Disruption from the C20 fatty acid ELO (C20ELO) and ∆four fatty acid DES (∆4DES) genes with expression of ω3 fatty acid DES Within this thraustochytrid authorized the production of EPA and n-3docosapentaenoic acid (n-3DPA), respectively, at the highest amount amid recognized microbial resources utilizing fed-batch society.
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